From stem cells to functional tissue
We differentiate human iPSCs into 3D pancreatic microtissues that replicate the structure and function of native human islets.
iPSC differentiation process
iPSC expansion
Human iPSCs are expanded under defined, feeder-free conditions to ensure consistent starting material.
Directed differentiation
A multi-stage protocol guides iPSCs through definitive endoderm, pancreatic progenitor, and endocrine precursor stages.
3D aggregation
Endocrine cells self-organize into 3D microtissues (pseudoislets) that mimic the architecture of native pancreatic islets.
Functional maturation
Mature microtissues exhibit glucose-stimulated insulin secretion (GSIS), the gold-standard functional readout.
How we compare
| 2D cell lines | Animal models | StemX microtissues | |
|---|---|---|---|
| Physiological relevance | Low | Moderate | High |
| Human translation | Poor | Poor (5%) | Direct |
| Reproducibility | High | Low | High |
| Throughput | High | Low | Medium-High |
| Ethical concerns | None | Significant | None |
| 3D architecture | No | Yes | Yes |
Applications
Drug screening
Test compound efficacy and toxicity on human pancreatic tissue. GSIS, viability, and multi-parameter readouts.
Therapy validation
Validate transduction efficiency and functional rescue in a human 3D tissue context. Includes toxicity assessment.
Disease modeling
Model metabolic disease phenotypes using patient-derived or gene-edited iPSC lines in a physiologically relevant format.
Want to learn more about our platform?
We are happy to walk you through our technology, discuss your application, or design a pilot study.